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Alkaline conditions are used to disrupt the vitamin B12 from the binding proteins, after which the vitamin B12 is generally converted to cyanocobalamin by potassium cyanide, prior to measurement (Klee, 2000)

Collectively, therefore, cell cycle exit and translational suppression were sufficient to protect ES cells from the lethal effects of Cys withdrawal, providing a common broad protective pathway for blocking death by Cys or Thr starvation

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Suppose your canonical site has kcat/KM of 10 Ms (typical for TEV on its preferred substrate)

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